Nematodes Guardian of Gardens

Nématodes parasites mortels pour sciarides et mouches terreaux

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Identification card icon Identification

Guardian of Gardens is a mixure of several species of entomopathogenic nematodes of the genus Steinernema and Heterorhabditis which attack several soil dwelling pests.

Entomopathogenic nematodes are distributed in the juvenile stage (infectious larvae). There are 0,4 to 1,5 mm long and can be observed with a 20 X magnifying glass. Once in the soil, they penetrate in the insect pest by its natural openings (mouth, respiratory orifices and anus). The Nematodes feed on the tissues and release symbiotic bacteria that are present in their intestines. These bacteria kill the host after 24 to 48 hours..

  • Vegetable (Market Garden)
  • Gardens

  • Rose chafer
  • Japanese beetle
  • June beetle
  • Vine weevil
  • Birch leafminer
  • Carrot fly

  • Optimum conditions are a temperature between 15 to 20°C and moist soil.
  • Become inactive below 15°C and above 30°C.
  • The ideal pH is between 4 to 8.
  • After their application, juvenile nematodes look for hosts to infect.
  • They enter the target insects through their natural orifices (mouth, anus and spiracles).
  • They then release a bacterium that kills the host within a few days.
  • Nematodes cause host death within days.


Sponge with nematodes

A synthetic sponge containing entomopathogenic nematodes. Just squeeze the sponge into the water to make the nematode solution.

Before starting

  • Always check the viability of your beneficial nematodes.
  • To do this, place a small amount of your nematode mixture in a transparent plastic bag (eg: ziploc bag) and observe the nematodes using a 20 X magnifying glass.
  • Live nematodes (0.4 to 1, 5 mm) squirm or are shaped like an “S”.
  • Dead nematodes are immobile and straight.

How to apply

Guardians of the Garden nematodes can be applied using conventional application equipment (including tank sprayers, backpack sprayers and injection irrigation systems).

  • Water the substrate abundantly before treating.
  • It should be very wet. Do not apply on a sunny day.
  • Do not mix with fertilizers and pesticides..
  • Clean your tank well to remove pesticide residues before putting your nematodes in it.
  • Remove filters from sprayer
  • Apply immediately after preparation, nematodes will not keep.
  • Keep the mixture stirring.

Application rate

Nematodes Guardian of Gardens
Formats Superficy
10 millions 2 000 - 3 000 pi2
50 million 10 000 pi2
100 million 20 000 pi2
250 million 1 acre
500 million 1 ha (2,5 acres)


  • Sponges can not be subdivided, however well mixed solution can be portioned.
  • Once a sponge is mixed with water into a suspension, use the entire suspension immediately.
  • Use cool/tepid water for mixing and keep tank out of direct sunlight.
  • Remove filters that are finer than 100 mesh from application equipment and nozzles.
  • Do not use in application systems where nozzle aperture is smaller than 0.149 mm (149 microns).
  • Do not exceed 300 PSI pump pressure.
  • Provide gentle agitation or aeration to the nematode solution during application.
  • Do not store extra product once mixed with water.

  • Upon receipt of product, remove from shipping packaging and use immediately or store in a refrigerated area 5 °C (40 °F) Do not freeze.
  • Do not freeze.
  • Do not use if incorrectly stored.
  • Store for a maximum of 6 weeks.
  • Always check viability before making the root application (see section Before starting)

  1.   - Biological Control: Using Beneficial Nematodes by University of Massachusetts Amherst (2015)

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